RNA

Part:BBa_K2240005:Design

Designed by: iGEM2017_HKUST   Group: iGEM17_Hong_Kong_HKUST   (2017-10-18)


Antisense RNA type 2 (Anti2) with Hfq binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NIL


Source

The sequences of the Hfq binding region and the antisense RNA type 2 were acquired from the research paper, Development of design rules for reliable antisense RNA behavior in E. coli. Only the sequence of the antisense RNA type 2 was later modified.


References

Hoynes-O’Connor, A., & Moon, T. S. (2016). Development of design rules for reliable antisense RNA behavior in E. coli. ACS synthetic biology, 5(12), 1441-1454.

Wagner, E. G. H. (2009). Kill the messenger: bacterial antisense RNA promotes mRNA decay. Nature structural & molecular biology, 16(8), 804-806.